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> Testosterone ELISA Kit

Testosterone ELISA Kit

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Cat# OKEH02537

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02537
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Testosterone ELISA Kit (OKEH02537) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Testosterone antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Testosterone and incubated. The Testosterone found in the sample or standards competes with the biotinylated Testosterone for limited binding sites on the immobilized anti-Testosterone antibody. Excess unbound biotinylated Testosterone and sample or standard Testosterone is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Testosterone captured in the well and inversely proportional to the amount of Testosterone which was contained in the sample or standard.
ELISA Kit Range3.9-250pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 100%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.8% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Testosterone Microplate96 Wells (12 x 8 Well strips)
Testosterone Lyophilized Standard2 x 10 ng
Sample Diluent1 x 20 mL
100X Testosterone-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: Testosterone
Target Name: Testosterone
::Pubchem: 6013
::Chemical formula: C19H28O2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity1.95 pg/mL
SpecificityNatural and recombinant General Testosterone
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsTestosterone, Testoderm, Sustanon, Testosteron, Androderm, Mertestate
Protein NameTestosterone
Description of TargetTestosterone is a steroid hormone from the androgen group and is found in mammals, reptiles, birds, and other vertebrates. In mammals, testosterone is primarily secreted in the testes of males and the ovaries of females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid.