Sulfadimethoxine ELISA Kit

Cat# OKAO00109

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Sulfadimethoxine ELISA Kit (OKAO00109)
Product Info
Predicted Species ReactivityGeneral
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology Sulfadimethoxine ELISA Kit (OKAO00109) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Sulfadimethoxine has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-sulfadimethoxine antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
ELISA Kit Range1-81 ppb
ELISA Kit Recovery
SampleAmount
Tissue85% +/- 10%
Honey95% +/- 10%
Serum90% +/- 10%
Milk95% +/- 10%
ELISA Kit ReproducibilityMean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
ELISA Kit Component
ComponentAmount
Sulfadimethoxine Microplate96 Wells (12 x 8 Well Strips)
Sulfadimethoxine Standard6 x 1 mL
Sulfadimethoxine Rabbit Detector Antibody1 x 7 mL
Anti-Rabbit / HRP Conjugate1 x 7 mL
Detection Reagent A1 x 7 mL
Detection Reagent B1 x 7 mL
2X Sample Prep Buffer2 x 20 mL
20X Wash Buffer2 x 20 mL
Stop Solution1 x 7 mL
Additional InformationCAS: 122-11-2 PubChem: 5323
::Molecular Formula: C12H14N4O4S
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue (meat, chicken, beef, rabbit, duck, liver, shrimp, fish, egg), serum, honey, milk
Sensitivity
SampleAmount
Tissue1 ppb
Honey1 ppb
Serum4 ppb
Milk20 ppb
Specificity
SubstanceCross Reactivity Rate
Sulfadimethoxine100%
Sulfadiazine168%
Sulfamethoxazole169%
Sulfamerazine200%
Sufachloropyridazine220%
Sulfamonomethoxine224%
Sulfamethoxypyridazine87%
Sulfathiazole78%
Sulfisomethazine112%
Sulfisomidine80%
Mafenide123%
Sulfamethoxydiazine134%
Sulfadimidine67%
Sulfafurazole240%
Pthalylsulfathizaole211%
Sulfaquinoxaline289%
Assay InfoAssay Methodology: Competitive Inhibition ELISA
Alias SymbolsSDM, Sulfadimethoxine, Sulphadimethoxine, 122-11-2, Sulfadimethoxydiazine, Sulfadimethoxin, Dimetazina
Protein NameSulfadimethoxine
Description of TargetSulfadimethoxine is a long-acting sulfonamide antibiotic used in veterinary medicine. Sulfadimethoxine inhibits bacterial synthesis of folic acid by competing with para-aminobenzoic acid (PABA) for the binding site on dihydropteroate synthase.
Molecular Weight310.32896 g/mol