Sphingosine ELISA Kit
Cat# OKEH02615
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Sphingosine ELISA Kit (OKEH02615) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Sphingosine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Sphingosine and incubated. The Sphingosine found in the sample or standards competes with the biotinylated Sphingosine for limited binding sites on the immobilized anti-Sphingosine antibody. Excess unbound biotinylated Sphingosine and sample or standard Sphingosine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Sphingosine captured in the well and inversely proportional to the amount of Sphingosine which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 107% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 6.6% (n = 20) Mean Inter-assay CV%: < 10.1% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: SPH Target Name: Sphingosine | ||||||||||||||||||||||
| :: | Pubchem: 5280335 | ||||||||||||||||||||||
| :: | Chemical formula: C18H37NO2 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.066 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General Sphingosine | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA | ||||||||||||||||||||||
| Description |
| Alias Symbols | SPH, Sphingosine, SPH |
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| Protein Name | Sphingosine |
| Description of Target | Sphingosine (2-amino-4-octadecene-1,3-diol) is an 18-carbon amino alcohol with an unsaturated hydrocarbon chain, which forms a primary part of sphingolipids, a class of cell membrane lipids that include sphingomyelin, an important phospholipid. |