Pyridinoline ELISA Kit

Cat# OKEH02582

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02582
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Pyridinoline ELISA Kit (OKEH02582) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Pyridinoline antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Pyridinoline and incubated. The Pyridinoline found in the sample or standards competes with the biotinylated Pyridinoline for limited binding sites on the immobilized anti-Pyridinoline antibody. Excess unbound biotinylated Pyridinoline and sample or standard Pyridinoline is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Pyridinoline captured in the well and inversely proportional to the amount of Pyridinoline which was contained in the sample or standard.
ELISA Kit Range6.25-400nmol/L
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Pyridinoline Microplate96 Wells (12 x 8 Well strips)
Pyridinoline Lyophilized Standard2 x 400 nmol
Sample Diluent1 x 20 mL
100X Pyridinoline-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: PYD
Target Name: Pyridinoline
::Pubchem: 105068
::Chemical formula: C18H28N4O8
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity3.125nmol/L
SpecificityNatural and recombinant General Pyridinoline
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsPYD, Pyridinoline, PYD
Protein NamePyridinoline
Description of TargetPyridinoline is a breakdown product of bone collagen. It can be used as a bone marker to measure osteoclastic activity.