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> Platelet Activating Factor ELISA Kit

Platelet Activating Factor ELISA Kit

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Cat# OKCD02282

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Platelet Activating Factor ELISA Kit (OKCD02282)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)78-105%85-101%98-105%91-101%
EDTA plasma(n=5)92-103%81-102%98-105%97-105%
heparin plasma(n=5)98-105%92-101%89-103%78-102%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Platelet Activating Factor (PAF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Platelet Activating Factor (PAF) and unlabeled Platelet Activating Factor (PAF) (Standards or samples) with the pre-coated antibody specific to Platelet Activating Factor (PAF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Platelet Activating Factor (PAF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Platelet Activating Factor (PAF) in the sample.
ELISA Kit Range37.0-3,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)79-9383
EDTA plasma(n=5)98-105102
heparin plasma(n=5)95-10398
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Activating Factor (PAF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Activating Factor (PAF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Platelet Activating Factor Microplate96 Wells (12 x 8 Well Strips)
Platelet Activating Factor Lyophilized Standard2
100X Platelet Activating Factor Biotin Complex120 uL
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Additional InformationCAS: 74389-68-7
PubChem: 108156
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity14.7 pg/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene SymbolPAF
Alias SymbolsPlatelet activating factor; C16-PAF; 1-O-Hexadecyl-2-acetyl-sn-glycero-3-phosphocholine; Hag-PC; 1-O-Hexadecyl-platelet-activating factor
Protein NamePlatelet Activating Factor