Phylloquinone ELISA Kit

Cat# OKEH02575

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Phylloquinone ELISA Kit (OKEH02575) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Phylloquinone antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Phylloquinone and incubated. The Phylloquinone found in the sample or standards competes with the biotinylated Phylloquinone for limited binding sites on the immobilized anti-Phylloquinone antibody. Excess unbound biotinylated Phylloquinone and sample or standard Phylloquinone is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Phylloquinone captured in the well and inversely proportional to the amount of Phylloquinone which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 7.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Phylloquinone Microplate96 Wells (12 x 8 Well strips)
Phylloquinone Lyophilized Standard2 x 10 ng
Sample Diluent1 x 20 mL
100X Phylloquinone-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: VK1
Target Name: Phylloquinone
::Pubchem: 5284607
::Chemical formula: C31H46O2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.05 ng/mL
SpecificityNatural and recombinant General Phylloquinone
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsVK1, Phylloquinone, VK1, Vitamin K1
Protein NamePhylloquinone
Description of TargetIt is often called vitamin K1, phytomenadione or phytonadione. Sometimes a distinction is made with phylloquinone considered natural and phytonadione considered synthetic. Phylloquinone is an electron acceptor during photosynthesis, forming part of the electron transport chain of Photosystem I. Its best-known function in animals is as a cofactor in the formation of coagulation factors II (prothrombin), VII, IX, and X by the liver. It is also required for the formation of anticoagulant factors protein C and S. It is commonly used to treat warfarin toxicity, and as an antidote for coumatetralyl. Vitamin K is also required for bone protein formation.