Penicillin G ELISA Kit
Cat# OKAO00124
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | General | ||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||
| ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Penicillin G ELISA Kit (OKAO00124) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Penicillin G has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Penicillin G antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample. | ||||||||||||||||||||
| ELISA Kit Range | 0.05-4.05 ppb | ||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Mean Intra-Assay: CV<8% (n= 3 x 20) Mean Inter-Assay: CV<12% (n= 3 x 40) | ||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | CAS: 69-57-8 PubChem: 23668834 | ||||||||||||||||||||
| :: | Molecular Formula: C16H17N2NaO4S | ||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||
| Sample Type | Tissue (chicken, duck, porcine meat, liver, shrimp, fish. etc), Honey, Milk. | ||||||||||||||||||||
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| Assay Info | Assay Methodology: Competitive Inhibition ELISA |
| Alias Symbols | benzyl penicillin, Penicillin G sodium, Benzylpenicillin sodium, Penicillin G sodium salt, Crystapen, 69-57-8, BENZYLPENICILLIN SODIUM SALT |
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| Protein Name | Penicillin G |
| Description of Target | Benzylpenicillin Sodium is the sodium salt form of benzylpenicillin, a semi-synthetic, broad-spectrum penicillin antibiotic with bactericidal activity. Benzylpenicillin sodium binds to and inactivates penicillin-binding proteins (PBP) located on the inner membrane of the bacterial cell wall. Inactivation of PBPs interferes with the cross-linkage of peptidoglycan chains necessary for bacterial cell wall strength and rigidity. This results in the weakening of the bacterial cell wall and causes cell lysis. |
| Molecular Weight | 356.371949 g/mol |