Normetanephrine ELISA Kit
Cat# OKWB10533
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for Normetanephrine ELISA Kit (OKWB10533) |
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| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | 2.5 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Normetanephrine ELISA Kit (OKEH00637) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Normetanephrine antibody and blocked. Sample or standards are added to the wells along with a fixed quantity of biotinylated Normetanephrine and incubated. The Normetanephrine found in the sample or standards competes with the biotinylated Normetanephrine for limited binding sites on the immobilized anti-Normetanephrine antibody. Excess unbound biotinylated Normetanephrine and sample or standard Normetanephrine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Normetanephrine captured in the well and inversely proportional to the amount of Normetanephrine which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 6.25-400pg/ml | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay Precision: 3 samples with known low, middle and high level Serping1Normetanephrineplicates, respectively. Intra-Assay CV: <10% Inter-Assay CV: <12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | CAS: 97-31-4 PubChem: 1237 Molecular Formula: C9H13NO3 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | < 3.75 ng/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive Inhibition ELISA |
| Alias Symbols | 4-(2-amino-1-hydroxy-ethyl)- 2-methoxy-phenol, NORMETANEPHRINE, Normetadrenaline, 4-(2-Amino-1-hydroxyethyl)-2-methoxyphenol, (+/-)-Normetanephrine |
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| Protein Name | Normetanephrine |
| Description of Target | Normetanephrine is a methylated metabolite of norepinephrine that is excreted in the urine and found in certain tissues. It is a marker for tumors. |
| Protein Size (# AA) | 183.204 g/mol |