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> Nitrofurazone ELISA Kit

Nitrofurazone ELISA Kit

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Cat# OKAO00102

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Nitrofurazone ELISA Kit (OKAO00102)
Product Info
Predicted Species ReactivityGeneral
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology Nitrofurazone ELISA Kit (OKAO00102) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Nitrofurazone has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Nitrofurazone antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
ELISA Kit Range0.05-4.05 ppb
ELISA Kit Recovery
SampleAmount
Tissue, Liver90% +/- 15%
Honey, milk80% +/- 15%
Intestine80% +/- 15%
ELISA Kit ReproducibilityMean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
ELISA Kit Component
ComponentAmount
Nitrofurazone Microplate96 Wells (12 x 8 Well Strips)
Nitrofurazone Standards6 x 1 mL
Nitrofurazone Rabbit Detector Antibody1 x 7 mL
Anti-Rabbit / HRP Conjugate1 x 7 mL
Detection Reagent A1 x 7 mL
Detection Reagent B1 x 7 mL
Stop Solution1 x 7 mL
20X Wash Buffer2 x 20 mL
2X Sample Prep Buffer2 x 20 mL
2-Nitrobenzaldehyde (C7H5NO3)1 x 10 mL
Additional InformationCAS: 59-87-0
PubChem: 5447130
::Molecular Formula: C6H6N4O4
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue (Chicken, duck, porcine meat, beef and mutton, fish, shrimp), liver (Chicken, duck, porcine meat, beef and mutton), honey, milk, intestine.
Sensitivity
SampleAmount
Tissue0.1 ppb
Liver0.1 ppb
Honey, milk0.1 ppb
Intestine0.1 ppb
Specificity
SubstanceCross Reactivity Rate
SEM100%
AHD<0.1%
AOZ<0.1%
AMOZ<0.1%
Assay InfoAssay Methodology: Competitive Inhibition ELISA
Alias SymbolsSEM, Nitrofurazone, Nitrofural, Furacilin, Furacin, 59-87-0, Furacillin
Protein NameNitrofurazone
Description of TargetA topical anti-infective agent effective against gram-negative and gram-positive bacteria. It is used for superficial WOUNDS AND INJURIES and skin infections. Nitrofurazone has also been administered orally in the treatment of TRYPANOSOMIASIS.
Molecular Weight198.13624 g/mol