Neomycin ELISA Kit

Cat# OKAO00126

Size : 96Wells

Brand : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Neomycin ELISA Kit (OKAO00126)
Product Info
Predicted Species ReactivityGeneral
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology Neomycin ELISA Kit (OKAO00126) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Neomycin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Neomycin antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
ELISA Kit Range0.5-40.5 ppb
ELISA Kit Recovery
SampleAmount
Tissue90% +/- 20%
Honey90% +/- 15%
ELISA Kit ReproducibilityMean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
ELISA Kit Component
ComponentAmount
Neomycin Microplate96 Wells (12 x 8 Well Strips)
Neomycin Standards5 x 1 mL
Neomycin Rabbit Detector Antibody1 x 7 mL
Anti-Rabbit / HRP Conjugate1 x 7 mL
Detection Reagent A1 x 7 mL
Detection Reagent B1 x 7 mL
Stop Solution1 x 7 mL
20X Wash Buffer1 x 20 mL
5X Sample Prep Buffer1 x 20 mL
Additional InformationCAS: 1405-10-3
PubChem: 71463900
::Molecular Formula: C23H48N6O17S
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue (chicken, duck, porcine meat, liver, shrimp, fish. etc), Honey.
Sensitivity
SampleAmount
Tissue30 ppb
Honey30 ppb
Specificity
SubstanceCross Reactivity Rate
Neomycin100%
Streptomycin<0.1%
Gentamicin<0.1%
Dihydrostreptomycin<0.1%
Kanamycin<0.1%
Tobramycin<0.1%
Sisomicin<0.1%
Assay InfoAssay Methodology: Competitive Inhibition ELISA
Alias SymbolsNeomycin, NEOMYCIN SULFATE, 1405-10-3, AKOS016010116, CS-O-01947, AK115669, SC-46589
Protein NameNeomycin
Description of TargetAntibiotic complex produced by Streptomyces fradiae. NEOMYCIN SULFATE is composed of neomycins A, B, and C. It acts by inhibiting translation during protein synthesis.
Molecular Weight712.72222 g/mol