Melamine ELISA Kit
Cat# OKAO00120
Size : 96Wells
Brand : Aviva Systems Biology
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Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | General | ||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||
| ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Melamine ELISA Kit (OKAO00120) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Melamine has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Melamine antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample. | ||||||||||||||||||||
| ELISA Kit Range | 1-81 ppb | ||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Mean Intra-Assay: CV<8% (n= 3 x 20) Mean Inter-Assay: CV<12% (n= 3 x 40) | ||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | CAS: 108-78-1 PubChem: 7955 | ||||||||||||||||||||
| :: | Molecular Formula: C3H6N6 or C3N3(NH2)3 | ||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||
| Sample Type | Milk powder, milk, tissue (chicken, duck, fish, shrimp, liver. etc), feed, egg and serum. | ||||||||||||||||||||
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| Assay Info | Assay Methodology: Competitive Inhibition ELISA |
| Alias Symbols | Melamine, MELAMINE, 1,3,5-Triazine-2,4,6-triamine, Cyanurotriamide, Cyanuramide, Cyanurotriamine, 108-78-1 |
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| Protein Name | Melamine |
| Description of Target | Melamine is an organic base and a trimer of cyanamide, with a 1,3,5-triazine skeleton. Like cyanamide, it contains 67% nitrogen by mass and, if mixed with resins, has fire retardant properties due to its release of nitrogen gas when burned or charred, and has several other industrial uses. Melamine is also a metabolite of cyromazine, a pesticide. It is formed in the body of mammals that have ingested cyromazine.[2] It has been reported that cyromazine can also be converted to melamine in plants.[ |
| Molecular Weight | 126.11994 g/mol |