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> Lumisterol ELISA Kit

Lumisterol ELISA Kit

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Cat# OKCD02278

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Lumisterol ELISA Kit (OKCD02278)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)98-105%88-97%80-88%98-105%
EDTA plasma(n=5)81-89%94-102%83-97%80-89%
heparin plasma(n=5)90-105%98-105%89-101%78-104%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Lumisterol (Lum) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Lumisterol (Lum) and unlabeled Lumisterol (Lum) (Standards or samples) with the pre-coated antibody specific to Lumisterol (Lum). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Lumisterol (Lum) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Lumisterol (Lum) in the sample.
ELISA Kit Range4.94-400ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)80-9586
EDTA plasma(n=5)92-10599
heparin plasma(n=5)80-9082
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lumisterol (Lum) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lumisterol (Lum) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Lumisterol Microplate96 Wells (12 x 8 Well Strips)
Lumisterol Lyophilized Standard2
Lumisterol Biotin Complex1
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Biotin Complex Reconstitution Buffer1 x 300 uL
Additional InformationCAS: 474-69-1
PubChem: 6436872
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity2.19 ng/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene SymbolLUM
Alias SymbolsLumisterol; 474-69-1; 9-beta,10-alpha-Ergosta-5,7,22-trien-3-beta-ol
Protein NameLumisterol
Description of TargetLumisterol is a steroid of interest both because its biosynthesis in FUNGI is a target of ANTIFUNGAL AGENTS, notably AZOLES, and because when it is present in SKIN of animals, ULTRAVIOLET RAYS break a bond to result in ERGOCALCIFEROL.