Lipopolysaccharide ELISA Kit

Cat# OKEH02594

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02594
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Lipopolysaccharide ELISA Kit (OKEH02594) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Lipopolysaccharide has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lipopolysaccharide is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lipopolysaccharide captured in the well.
ELISA Kit Range7.8-500 pg/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.4% (n = 20)
Mean Inter-assay CV%: < 8.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Lipopolysaccharide Microplate96 Wells (12 x 8 Well strips)
Lipopolysaccharide Lyophilized Standard2 x 5 EU
Sample Diluent1 x 20 mL
100X Biotinylated Lipopolysaccharide Detector Ab1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: LPS
Target Name: Lipopolysaccharide
::Pubchem: 11970143
::Chemical formula: C205H366N3O117P5
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity3.5 pg/mL
SpecificityNatural and recombinant General Lipopolysaccharide
Publications

Rhubarb Enema Improved Colon Mucosal Barrier Injury in 5/6 Nephrectomy Rats May Associate With Gut Microbiota Modification. Front Pharmacol. 11, 1092 (2020). 32848732

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Alias SymbolsLPS, Lipopolysaccharide, LPS
Protein NameLipopolysaccharide
Description of TargetLipopolysaccharides (LPS), also known as lipoglycans, are large molecules consisting of a lipid and a polysaccharide joined by a covalent bond; they are found in the outer membrane of Gram-negative bacteria, act as endotoxins and elicit strong immune responses in animals.

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