Lipoarabinomannan ELISA Kit
Cat# OKEH02596
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Lipoarabinomannan ELISA Kit (OKEH02596) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Lipoarabinomannan antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Lipoarabinomannan and incubated. The Lipoarabinomannan found in the sample or standards competes with the biotinylated Lipoarabinomannan for limited binding sites on the immobilized anti-Lipoarabinomannan antibody. Excess unbound biotinylated Lipoarabinomannan and sample or standard Lipoarabinomannan is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Lipoarabinomannan captured in the well and inversely proportional to the amount of Lipoarabinomannan which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 89% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 6.0% (n = 20) Mean Inter-assay CV%: < 9.9% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: LAM Target Name: Lipoarabinomannan | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.101 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General Lipoarabinomannan | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA | ||||||||||||||||||||||
| Description |
| Alias Symbols | LAM, Lipoarabinomannan, LAM |
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| Protein Name | Lipoarabinomannan |
| Description of Target | Lipoarabinomannan is synthesized when a mannopyranosyl adds to a phosphoinositol to produce PIMs and lipomannan(LM). PIM and LM are then glycosylated with arabinan to form LAM. Lipoarabinomannan, also called LAM, is a glycolipid, and a virulence factor associated with Mycobacterium tuberculosis, the bacteria responsible for tuberculosis. Its primary function is to inactivate macrophages and scavenge oxidative radicals. |