Keratan sulfate ELISA Kit
Cat# OKEH02547
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Keratan sulfate ELISA Kit (OKEH02547) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Keratan sulfate antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Keratan sulfate and incubated. The Keratan sulfate found in the sample or standards competes with the biotinylated Keratan sulfate for limited binding sites on the immobilized anti-Keratan sulfate antibody. Excess unbound biotinylated Keratan sulfate and sample or standard Keratan sulfate is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Keratan sulfate captured in the well and inversely proportional to the amount of Keratan sulfate which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 97% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.3% (n = 20) Mean Inter-assay CV%: < 7.5% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: KS Target Name: Keratan sulfate | ||||||||||||||||||||||
| :: | Pubchem: 446715 | ||||||||||||||||||||||
| :: | Chemical formula: C28H48N2O32S4 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.089 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General Keratan sulfate | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Alias Symbols | KS, Keratan sulfate, KS |
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| Protein Name | Keratan sulfate |
| Description of Target | Keratan sulfate (KS), also called keratosulfate, is any of several sulfated glycosaminoglycans (structural carbohydrates) that have been found especially in the cornea, cartilage, and bone. It is also synthesized in the central nervous system where it participates both in development and in the glial scar formation following an injury. Keratan sulfates are large, highly hydrated molecules which in joints can act as a cushion to absorb mechanical shock. |