Florfenicol ELISA Kit

Cat# OKAO00131

Size : 96Wells

Brand : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Florfenicol ELISA Kit (OKAO00131)
Product Info
Predicted Species ReactivityGeneral
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology Florfenicol ELISA Kit (OKAO00131) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Florfenicol has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Florfenicol antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
ELISA Kit Range1-81 ppb
ELISA Kit Recovery
SampleAmount
Tissue85% +/- 10%
Liver85% +/- 10%
Honey80% +/- 10%
ELISA Kit ReproducibilityMean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
ELISA Kit Component
ComponentAmount
Florfenicol Microplate96 Wells (12 x 8 Well Strips)
Florfenicol Standards6 x 1 mL
Florfenicol Rabbit Detector Antibody1 x 7 mL
Anti-Rabbit / HRP Conjugate1 x 12 mL
Detection Reagent A1 x 7 mL
Detection Reagent B1 x 7 mL
Stop Solution1 x 7 mL
20X Wash Buffer2 x 20 mL
2X Sample Prep Buffer2 x 20 mL
Additional InformationCAS: 73231-34-2
PubChem: 114811
::Molecular Formula: C12H14Cl2FNO4S
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue (Chicken, duck, porcine meat, beef and mutton, fish, shrimp), liver (Chicken, duck, porcine meat, beef and mutton), honey.
Sensitivity
SampleAmount
Tissue1 ppb
Liver1 ppb
Honey1 ppb
Specificity
SubstanceCross Reactivity Rate
Florfeniol100%
Florfenicol amine15%
Thiamphenicol<0.1%
Chloramphenicol<0.1%
Assay InfoAssay Methodology: Competitive Inhibition ELISA
Alias SymbolsFF, Florfenicol, 73231-34-2, Nuflor, Sch-25298, (-)-Florfenicol, Aquafen
Protein NameFlorfenicol
Description of TargetFlorfenicol is a fluorinated synthetic analog of thiamphenicol, mainly used in veterinary medicine.
Molecular Weight358.213263 g/mol