Enrofloxacin ELISA Kit

Cat# OKAO00116

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Enrofloxacin ELISA Kit (OKAO00116)
Product Info
Predicted Species ReactivityGeneral
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology Enrofloxacin ELISA Kit (OKAO00116) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Enrofloxacin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Enrofloxacin antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
ELISA Kit Range0.1-8.1 ppb
ELISA Kit Recovery
SampleAmount
Tissue85% +/- 10%
Honey85% +/- 10%
ELISA Kit ReproducibilityMean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
ELISA Kit Component
ComponentAmount
Enrofloxacin Microplate96 Wells (12 x 8 Well Strips)
Enrofloxacin Standards6 x 1 mL
Enrofloxacin Rabbit Detector Antibody1 x 7 mL
Anti-Rabbit / HRP Conjugate1 x 7 mL
Detection Reagent A1 x 7 mL
Detection Reagent B1 x 7 mL
Stop Solution1 x 7 mL
20X Wash Buffer2 x 20 mL
2X Sample Prep Buffer3 x 15 mL
Additional InformationCAS: 93106-60-6
PubChem: 71188
::Molecular Formula: C19H22FN3O3
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissues (chicken, shrimp, fish, liver, etc) and honey.
Sensitivity
SampleAmount
Tissue1 ppb
Honey1 ppb
Specificity
SubstanceCross Reactivity Rate
Enrofloxacin100%
Ciprofloxacin3.8%
Norfloxacin2.4%
Sarafloxacin1.8%
Assay InfoAssay Methodology: Competitive Inhibition ELISA
Alias SymbolsENR, Enrofloxacin, Baytril, 93106-60-6, Enrofloxacine, CFPQ, Bay-Vp-2674
Protein NameEnrofloxacin
Description of TargetEnrofloxacin (ENR) is a fluoroquinolone antibiotic sold by the Bayer Corporation under the trade name Baytril. Enrofloxacin is currently approved by the FDA for the treatment of individual pets and domestic animals in the United States. In September 2005, the FDA withdrew approval of Baytril for use in water to treat flocks of poultry, as this practice was noted to promote the evolution of fluoroquinolone-resistant strains of the bacterium Campylobacter, a human pathogen.
Molecular Weight359.394683 g/mol