Enrofloxacin ELISA Kit
Cat# OKAO00116
Size : 96Wells
Brand : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for Enrofloxacin ELISA Kit (OKAO00116) |
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Predicted Species Reactivity | General | ||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Enrofloxacin ELISA Kit (OKAO00116) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Enrofloxacin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Enrofloxacin antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample. | ||||||||||||||||||||
ELISA Kit Range | 0.1-8.1 ppb | ||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay: CV<8% (n= 3 x 20) Mean Inter-Assay: CV<12% (n= 3 x 40) | ||||||||||||||||||||
ELISA Kit Component |
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Additional Information | CAS: 93106-60-6 PubChem: 71188 | ||||||||||||||||||||
:: | Molecular Formula: C19H22FN3O3 | ||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||
Sample Type | Tissues (chicken, shrimp, fish, liver, etc) and honey. | ||||||||||||||||||||
Sensitivity |
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Specificity |
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Assay Info | Assay Methodology: Competitive Inhibition ELISA |
Alias Symbols | ENR, Enrofloxacin, Baytril, 93106-60-6, Enrofloxacine, CFPQ, Bay-Vp-2674 |
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Protein Name | Enrofloxacin |
Description of Target | Enrofloxacin (ENR) is a fluoroquinolone antibiotic sold by the Bayer Corporation under the trade name Baytril. Enrofloxacin is currently approved by the FDA for the treatment of individual pets and domestic animals in the United States. In September 2005, the FDA withdrew approval of Baytril for use in water to treat flocks of poultry, as this practice was noted to promote the evolution of fluoroquinolone-resistant strains of the bacterium Campylobacter, a human pathogen. |
Molecular Weight | 359.394683 g/mol |