Reagents and instruments for immunology, cell biology and molecular biology.
 
> Dopamine ELISA Kit

Dopamine ELISA Kit

Print Print
Please log in

Cat# OKCD02264

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Dopamine ELISA Kit (OKCD02264)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)94-103%94-105%97-104%85-104%
EDTA plasma(n=5)78-102%92-105%80-99%79-101%
heparin plasma(n=5)91-99%85-103%78-93%80-101%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Dopamine (DA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Dopamine (DA) and unlabeled Dopamine (DA) (Standards or samples) with the pre-coated antibody specific to Dopamine (DA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Dopamine (DA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Dopamine (DA) in the sample.
ELISA Kit Range12.35-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)95-10299
EDTA plasma(n=5)86-9389
heparin plasma(n=5)90-9994
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopamine (DA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopamine (DA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Dopamine Microplate96 Wells (12 x 8 Well Strips)
Dopamine Lyophilized Standard2
100X Dopamine Biotin Complex120 uL
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Additional InformationCAS: 51-61-6
PubChem: 681
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity4.71 pg/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene SymbolDA
Alias SymbolsDopamine; 4-(2-Aminoethyl)benzene-1,2-diol; Hydroxytyramin; Oxytyramine; Dopamin
Protein NameDopamine
Description of TargetOne of the catecholamine NEUROTRANSMITTERS in the brain. Dopamine is derived from TYROSINE and is the precursor to NOREPINEPHRINE and EPINEPHRINE. Dopamine is a major transmitter in the extrapyramidal system of the brain, and important in regulating movement. A family of receptors (RECEPTORS, DOPAMINE) mediate its action.