Diethylstilbestrol ELISA Kit

Cat# OKAO00122

Size : 96Wells

Brand : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Diethylstilbestrol ELISA Kit (OKAO00122)
Product Info
Predicted Species ReactivityGeneral
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology Diethylstilbestrol ELISA Kit (OKAO00122) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Diethylstilbestrol has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Diethylstilbestrol antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
ELISA Kit Range0.025-2.025 ppb
ELISA Kit Recovery
SampleAmount
Urine70% +/- 10%
Feed90% +/- 10%
Tissue85% +/- 10%
ELISA Kit ReproducibilityMean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
ELISA Kit Component
ComponentAmount
Diethylstilbestrol Microplate96 Wells (12 x 8 Well Strips)
Diethylstilbestrol Standards6 x 1 mL
Diethylstilbestrol Rabbit Detector Antibody1 x 7 mL
Anti-Rabbit / HRP Conjugate1 x 12 mL
Detection Reagent A1 x 7 mL
Detection Reagent B1 x 7 mL
Stop Solution1 x 7 mL
20X Wash Buffer2 x 20 mL
5X Sample Prep Buffer2 x 20 mL
Additional InformationCAS: 56-53-1
PubChem: 448537
::Molecular Formula: C18H20O2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeFeed, meat, liver (Chicken, duck, porcine meat, beef and mutton), shrimp, fish, urine
Sensitivity
SampleAmount
Shrimp, fish0.2 ppb
Liver2 ppb
Urine0.6 ppb
Feed20 ppb
Specificity
SubstanceCross Reactivity Rate
DES100%
Dienestrol38.5%
Hexestrol8.5%
Ethinylestradiol<0.1%
Estriol<0.1%
Assay InfoAssay Methodology: Competitive Inhibition ELISA
Alias SymbolsDES, Diethylstilbestrol, Stilbestrol, 56-53-1, Stilboestrol, Distilbene, Stilboestroform
Protein NameDiethylstilbestrol
Description of TargetDiethylstilbestrol is a synthetic, nonsteroidal form of estrogen. A well-known teratogen and carcinogen, diethylstilbestrol inhibits the hypothalamic-pituitary-gonadal axis, thereby blocking the testicular synthesis of testosterone, lowering plasma testosterone, and inducing a chemical castration.
Molecular Weight268.3502 g/mol