Creatinine ELISA Kit

Cat# OKEH02617

Size : 96Wells

Brand : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02617
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Creatinine ELISA Kit (OKEH02617) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Creatinine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Creatinine and incubated. The Creatinine found in the sample or standards competes with the biotinylated Creatinine for limited binding sites on the immobilized anti-Creatinine antibody. Excess unbound biotinylated Creatinine and sample or standard Creatinine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Creatinine captured in the well and inversely proportional to the amount of Creatinine which was contained in the sample or standard.
ELISA Kit Range2.8-180umol/L
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.5% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Creatinine Microplate96 Wells (12 x 8 Well strips)
Creatinine Lyophilized Standard2 x 180 umol
Sample Diluent1 x 20 mL
100X Creatinine-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: Cr
Target Name: Creatinine
::Pubchem: 588
::Chemical formula: C4H7N3O
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity1.4umol/ml
SpecificityNatural and recombinant General Creatinine
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsCr, Creatinine, Cr
Protein NameCreatinine
Description of TargetMeasuring serum creatinine is a simple test and it is the most commonly used indicator of renal function. In the United States, creatinine is typically reported in mg/dL, whereas, in Canada and a few European countries, umol/litre may be used. 1 mg/dL of creatinine is 88.4 umol/L. The typical human reference ranges for serum creatinine are 0.5 to 1.0 mg/dL (about 45-90 umol/L) for women and 0.7 to 1.2 mg/dL (60-110 umol/L) for men. While a baseline (medicine) serum creatinine of 2.0 mg/dL (150 umol/L) may indicate normal kidney function in a male body builder, a serum creatinine of 1.2 mg/dL (110 umol/L) can indicate significant renal disease in an elderly female. for male reference range are 60-120 umol/L and for female it is 50-110 umol/L. Creatinine is a break-down product of creatine phosphate in muscle, and is usually produced at a fairly constant rate by the body (depending on muscle mass).

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