Cortisone ELISA Kit

Cat# OKEH02619

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Cortisone ELISA Kit (OKEH02619) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Cortisone antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Cortisone and incubated. The Cortisone found in the sample or standards competes with the biotinylated Cortisone for limited binding sites on the immobilized anti-Cortisone antibody. Excess unbound biotinylated Cortisone and sample or standard Cortisone is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Cortisone captured in the well and inversely proportional to the amount of Cortisone which was contained in the sample or standard.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Cortisone Microplate96 Wells (12 x 8 Well strips)
Cortisone Lyophilized Standard2 x 50 ng
Sample Diluent1 x 20 mL
100X Cortisone-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: CRO
Target Name: Cortisone
::Pubchem: 222786
::Chemical formula: C21H28O5
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.24 ng/mL
SpecificityNatural and recombinant General Cortisone
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsCRO, Cortisone, CRO
Protein NameCortisone
Description of TargetCortisone (17-hydroxy-11-dehydrocorticosterone) is a steroid hormone. It is one of the main hormones released by the adrenal gland in response to stress. In chemical structure, it is a corticosteroid closely related to corticosterone. It is used to treat a variety of ailments and can be administered intravenously, orally, intraarticularly, or transcutaneously. Cortisone suppresses the immune system, thus reducing inflammation and attendant pain and swelling at the site of the injury. Risks exist, in particular in the long-term use of cortisone.

You might also be interested by the following products:



Cat#
Description
Cond.
Price Bef. VAT
KA3403
 1Kit