Ciprofloxacin ELISA Kit
Cat# OKAO00115
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Predicted Species Reactivity | General | ||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Ciprofloxacin ELISA Kit (OKAO00115) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Ciprofloxacin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Ciprofloxacin antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample. | ||||||||||||||||||||||
| ELISA Kit Range | 0.1-8.1 ppb | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Mean Intra-Assay: CV<8% (n= 3 x 20) Mean Inter-Assay: CV<12% (n= 3 x 40) | ||||||||||||||||||||||
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| Additional Information | CAS: 85721-33-1 PubChem: 2764 | ||||||||||||||||||||||
| :: | Molecular Formula: C17H18FN3O3 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Tissues (chicken, shrimp, fish, etc) and honey | ||||||||||||||||||||||
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| Assay Info | Assay Methodology: Competitive Inhibition ELISA |
| Alias Symbols | CPFX, Ciprofloxacin, Ciprobay, 85721-33-1, Ciproxan, Ciprofloxacine, Ciprinol |
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| Protein Name | Ciprofloxacin |
| Description of Target | Ciprofloxacin is a synthetic broad spectrum fluoroquinolone antibiotic. Ciprofloxacin binds to and inhibits bacterial DNA gyrase, an enzyme essential for DNA replication. This agent is more active against Gram-negative bacteria than Gram-positive bacteria. |
| Molecular Weight | 331.341523 g/mol |