Cholesterol ELISA Kit
Cat# OKEH02598
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Cholesterol ELISA Kit (OKEH02598) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Cholesterol antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Cholesterol and incubated. The Cholesterol found in the sample or standards competes with the biotinylated Cholesterol for limited binding sites on the immobilized anti-Cholesterol antibody. Excess unbound biotinylated Cholesterol and sample or standard Cholesterol is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Cholesterol captured in the well and inversely proportional to the amount of Cholesterol which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10nmol/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 112% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 6.9% (n = 20) Mean Inter-assay CV%: < 10.1% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: CH Target Name: Cholesterol | ||||||||||||||||||||||
| :: | Pubchem: 5997 | ||||||||||||||||||||||
| :: | Chemical formula: C27H46O | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.068nmol/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General Cholesterol | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Alias Symbols | CH, Cholesterol, CH |
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| Protein Name | Cholesterol |
| Description of Target | Cholesterol, from the Greek chole- (bile) and stereos (solid) followed by the chemical suffix -ol for an alcohol, is an organic chemical substance classified as a waxy steroid of fat. It is an essential structural component of mammalian cell membranes and is required to establish proper membrane permeability and fluidity. In addition to its importance within cells, cholesterol is an important component in the hormonal systems of the body for the manufacture of bile acids, steroid hormones, and vitamin D. Cholesterol is the principal sterol synthesized by animals; in vertebrates it is formed predominantly in the liver. Small quantities are synthesized in other cellular organisms (eukaryotes) such as plants and fungi. It is almost completely absent among prokaryotes, i.e. bacteria. Although cholesterol is important and necessary for human health, high levels of cholesterol in the blood have been linked to damage to arteries and cardiovascular disease. |