CdiGMP ELISA Kit (General)
Cat# OKEH04792
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
|---|
| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology CDIGMP ELISA Kit (General) (OKEH04792) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-general CDIGMP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CDIGMP and incubated. The CDIGMP found in the sample or standards competes with the biotinylated CDIGMP for limited binding sites on the immobilized anti-general CDIGMP antibody. Excess unbound biotinylated CDIGMP and sample or standard CDIGMP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CDIGMP captured in the well and inversely proportional to the amount of CDIGMP which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.78-50ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Spike Average Recovery: 101% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-Assay CV: < 4.6% Mean Inter-Assay CV: < 7.5% | ||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||
| Additional Information | CAS Number: 61093-23-0 Chemical Formula: C20H24N10O14P2 PubChem: 6323195 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids. | ||||||||||||||||||||||
| Sensitivity | 0.22 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General Cyclic Diguanylate | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Gene Symbol | CDIGMP |
|---|---|
| Molecular Weight | kDa |