Beta-Agonists ELISA Kit

Cat# OKAO00135

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Beta-Agonists ELISA Kit (OKAO00135)
Product Info
Predicted Species ReactivityGeneral
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology Beta-Agonists ELISA Kit (OKAO00135) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Beta-Agonists has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Beta-Agonists antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
ELISA Kit Range0.05-4.05 ppb
ELISA Kit Recovery
SampleAmount
Urine82% +/- 10%
Tissue (1)85% +/- 10%
Tissue (2)75% +/- 10%
Feed80% +/- 10%
ELISA Kit ReproducibilityMean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
ELISA Kit Component
ComponentAmount
Beta-Agonists Microplate96 Wells (12 x 8 Well Strips)
Beta-Agonists Standards6 x 1 mL
Beta-Agonists Rabbit Detector Antibody1 x 6 mL
Anti-Rabbit / HRP Conjugate1 x 700 uL
Detection Reagent A1 x 7 mL
Detection Reagent B1 x 7 mL
Stop Solution1 x 7 mL
20X Wash Buffer2 x 20 mL
5X Sample Prep Buffer1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeUrine, tissue, feed
Sensitivity
SampleAmount
Urine0.3 ppb
Tissue (1)0.1 ppb
Tissue (2)0.3 ppb
Feed5 ppb
Specificity
SubstanceCross Reactivity Rate
Beta-Agonists100%
Salbutamol100%
Cimbuterol109%
Clenbuterol130%
Tulobuterol50%
Terbutaline100%
Bambuterol24%
Brombuterol370%
Pirbuterol21%
Brombuterol hydrochloride354%
Clenproperol13%
Mabuterol160%
Cimaterol8%
Mapenterol322%
Clorprenaline3%
Clenpenterol229%
Ractopamine0.1%
Assay InfoAssay Methodology: Competitive Inhibition ELISA
Alias SymbolsBeta-Agonists
Protein NameBeta-Agonists
Description of TargetBeta agonists are medications that relax muscles of the airways, which widens the airways and results in easier breathing. They are a class of sympathomimetic agents which act upon the beta adrenoceptors. In general, pure beta-adrenergic agonists have the opposite function of beta blockers. Beta adrenoreceptor agonist ligands mimic the action of epinephrine and norepinephrine signaling in the heart, lungs and smooth muscle tissue, with epinephrine expressing the highest affinity.