Asymmetric dimethylarginine ELISA Kit
Cat# OKEH02587
Size : 96Wells
Brand : Aviva Systems Biology
Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Asymmetric dimethylarginine ELISA Kit (OKEH02587) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Asymmetric dimethylarginine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Asymmetric dimethylarginine and incubated. The Asymmetric dimethylarginine found in the sample or standards competes with the biotinylated Asymmetric dimethylarginine for limited binding sites on the immobilized anti-Asymmetric dimethylarginine antibody. Excess unbound biotinylated Asymmetric dimethylarginine and sample or standard Asymmetric dimethylarginine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Asymmetric dimethylarginine captured in the well and inversely proportional to the amount of Asymmetric dimethylarginine which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 15.6-1000ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | < 6.1% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 5.9% (n = 20) Mean Inter-assay CV%: < 7.4% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Target Abbr: ADMA Target Name: Asymmetric dimethylarginine | ||||||||||||||||||||||
:: | Pubchem: 501 | ||||||||||||||||||||||
:: | Chemical formula: C8H18N4O2 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
Sensitivity | 5.4 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant General Asymmetric dimethylarginine | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Alias Symbols | ADMA, Asymmetric dimethylarginine, ADMA |
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Protein Name | Asymmetric dimethylarginine |
Description of Target | Asymmetric dimethylarginine (ADMA) is a naturally occurring chemical found in blood plasma. It is a metabolic by-product of continual protein modification processes in the cytoplasm of all human cells. It is closely related to L-arginine, a conditionally-essential amino acid. ADMA interferes with L-arginine in the production of nitric oxide, a key chemical involved in normal endothelial function and, by extension, cardiovascular health. |