Asymmetric dimethylarginine ELISA Kit

Cat# OKEH02587

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02587
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Asymmetric dimethylarginine ELISA Kit (OKEH02587) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Asymmetric dimethylarginine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Asymmetric dimethylarginine and incubated. The Asymmetric dimethylarginine found in the sample or standards competes with the biotinylated Asymmetric dimethylarginine for limited binding sites on the immobilized anti-Asymmetric dimethylarginine antibody. Excess unbound biotinylated Asymmetric dimethylarginine and sample or standard Asymmetric dimethylarginine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Asymmetric dimethylarginine captured in the well and inversely proportional to the amount of Asymmetric dimethylarginine which was contained in the sample or standard.
ELISA Kit Range15.6-1000ng/mL
ELISA Kit Recovery< 6.1%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.9% (n = 20)
Mean Inter-assay CV%: < 7.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Asymmetric dimethylarginine Microplate96 Wells (12 x 8 Well strips)
Asymmetric dimethylarginine Lyophilized Standard2 x 1,000 ng
Sample Diluent1 x 20 mL
100X Asymmetric dimethylarginine-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: ADMA
Target Name: Asymmetric dimethylarginine
::Pubchem: 501
::Chemical formula: C8H18N4O2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity5.4 ng/mL
SpecificityNatural and recombinant General Asymmetric dimethylarginine
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsADMA, Asymmetric dimethylarginine, ADMA
Protein NameAsymmetric dimethylarginine
Description of TargetAsymmetric dimethylarginine (ADMA) is a naturally occurring chemical found in blood plasma. It is a metabolic by-product of continual protein modification processes in the cytoplasm of all human cells. It is closely related to L-arginine, a conditionally-essential amino acid. ADMA interferes with L-arginine in the production of nitric oxide, a key chemical involved in normal endothelial function and, by extension, cardiovascular health.

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Cat#
Description
Cond.
Price Bef. VAT
OKEH02612
 96Wells