Arginine vasopressin ELISA Kit
Cat# OKEH02585
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Arginine vasopressin ELISA Kit () (OKEH02585) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Arginine vasopressin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Arginine vasopressin and incubated. The Arginine vasopressin found in the sample or standards competes with the biotinylated Arginine vasopressin for limited binding sites on the immobilized anti-Arginine vasopressin antibody. Excess unbound biotinylated Arginine vasopressin and sample or standard Arginine vasopressin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Arginine vasopressin captured in the well and inversely proportional to the amount of Arginine vasopressin which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 31.2-2000pg/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.1% (n = 20) Mean Inter-assay CV%: < 7.5% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: AVPP Target Name: Arginine vasopressin | ||||||||||||||||||||||
| :: | Pubchem: 644077 | ||||||||||||||||||||||
| :: | Chemical formula: C46H65N15O12S2 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 15.1 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General Arginine vasopressin | ||||||||||||||||||||||
| Publications | Timeline of Changes in Biomarkers Associated with Spinal Cord Injury-Induced Polyuria. Neurotrauma Rep. 2, 462-475 (2021). 34901942 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA | ||||||||||||||||||||||
| Description |
| Alias Symbols | ARGIPRESSIN; 113-79-1; Arginine vasopressin; Argipressine; Pitressin; Vasopressin (arginine form) |
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| Protein Name | Arginine vasopressin |
| Description of Target | Vasopressin is the predominant form of mammalian antidiuretic hormone. It is a nonapeptide containing an arginine at residue 8 and two disulfide-linked cysteines at residues of 1 and 6. Arg-vasopressin is used to treat diabetes insipidus or to improve vasomotor tone and blodd pressure. |