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> Arginine ELISA Kit

Arginine ELISA Kit

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Cat# OKCD02224

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Arginine ELISA Kit (OKCD02224)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)81-93%78-95%78-90%92-99%
EDTA plasma(n=5)85-99%80-92%88-96%97-105%
heparin plasma(n=5)84-91%86-101%80-103%99-105%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Arginine (Arg) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Arginine (Arg) and unlabeled Arginine (Arg) (Standards or samples) with the pre-coated antibody specific to Arginine (Arg). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Arginine (Arg) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Arginine (Arg) in the sample.
ELISA Kit Range1.23-100ug/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)80-8985
EDTA plasma(n=5)82-9187
heparin plasma(n=5)78-9782
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arginine (Arg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arginine (Arg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Arginine Microplate96 Wells (12 x 8 Well Strips)
Arginine Lyophilized Standard2
Arginine Biotin Complex1
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Biotin Complex Reconstitution Buffer1 x 300 uL
Additional InformationCAS: 7200-25-1
PubChem: 232
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.51 ug/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene SymbolARG
Alias SymbolsDL-Arginine; 7200-25-1; Arginin; 2-amino-5-guanidinopentanoic acid
Protein NameArginine