Arginine ELISA Kit
Cat# OKEH02603
Size : 96Wells
Brand : Aviva Systems Biology
Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Arginine ELISA Kit (OKEH02603) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Arginine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Arginine and incubated. The Arginine found in the sample or standards competes with the biotinylated Arginine for limited binding sites on the immobilized anti-Arginine antibody. Excess unbound biotinylated Arginine and sample or standard Arginine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Arginine captured in the well and inversely proportional to the amount of Arginine which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 3.12-200umol/L | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.2% (n = 20) Mean Inter-assay CV%: < 7.3% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Target Abbr: ARG Target Name: Arginine | ||||||||||||||||||||||
:: | Pubchem: 6322 Chemical formula: C6H14N4O2 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
Sensitivity | 1.24umol/L | ||||||||||||||||||||||
Specificity | Natural and recombinant General Arginine | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA | ||||||||||||||||||||||
Description |
Alias Symbols | ARG, Arginine, ARG |
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Protein Name | Arginine |
Description of Target | Arginine (abbreviated as Arg or R) is an alpha-amino acid. It was first isolated in 1886. The L-form is one of the 20 most common natural amino acids. At the level of molecular genetics, in the structure of the messenger ribonucleic acid mRNA, CGU, CGC, CGA, CGG, AGA, and AGG, are the triplets of nucleotide bases or codons that codify for arginine during protein synthesis. In mammals, arginine is classified as a semiessential or conditionally essential amino acid, depending on the developmental stage and health status of the individual. Preterm infants are unable to synthesize or create arginine internally, making the amino acid nutritionally essential for them. |