Arginine ELISA Kit

Cat# OKEH02603

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02603
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Arginine ELISA Kit (OKEH02603) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Arginine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Arginine and incubated. The Arginine found in the sample or standards competes with the biotinylated Arginine for limited binding sites on the immobilized anti-Arginine antibody. Excess unbound biotinylated Arginine and sample or standard Arginine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Arginine captured in the well and inversely proportional to the amount of Arginine which was contained in the sample or standard.
ELISA Kit Range3.12-200umol/L
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 7.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Arginine Microplate96 Wells (12 x 8 Well strips)
Arginine Lyophilized Standard2 x 200 nmol
Sample Diluent1 x 20 mL
100X Arginine-Biotin Complex1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: ARG
Target Name: Arginine
::Pubchem: 6322
Chemical formula: C6H14N4O2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity1.24umol/L
SpecificityNatural and recombinant General Arginine
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Description
Alias SymbolsARG, Arginine, ARG
Protein NameArginine
Description of TargetArginine (abbreviated as Arg or R) is an alpha-amino acid. It was first isolated in 1886. The L-form is one of the 20 most common natural amino acids. At the level of molecular genetics, in the structure of the messenger ribonucleic acid mRNA, CGU, CGC, CGA, CGG, AGA, and AGG, are the triplets of nucleotide bases or codons that codify for arginine during protein synthesis. In mammals, arginine is classified as a semiessential or conditionally essential amino acid, depending on the developmental stage and health status of the individual. Preterm infants are unable to synthesize or create arginine internally, making the amino acid nutritionally essential for them.

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