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> 5-Methyltetrahydrofolate ELISA Kit

5-Methyltetrahydrofolate ELISA Kit

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Cat# OKCD02251

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for 5-Methyltetrahydrofolate ELISA Kit (OKCD02251)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)87-96%80-95%98-105%93-102%
EDTA plasma(n=5)81-102%91-104%85-92%96-103%
heparin plasma(n=5)99-105%93-101%96-105%84-95%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 5-Me-THF has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 5-Me-THF analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Me-THF in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 5-Me-THF in the sample.
ELISA Kit Range1.11-90ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)84-9992
EDTA plasma (n=5)96-105102
Heparin Plasma (n=5)80-10293
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Methyltetrahydrofolate (5-Me-THF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Methyltetrahydrofolate (5-Me-THF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-5-Methyltetrahydrofolate Microplate96 Wells (12 x 8 Well Strips)
5-Methyltetrahydrofolate Lyophilized Standard2
100X 5-Methyltetrahydrofolate Biotin Complex120 uL
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Additional InformationCAS: 134-35-0
PubChem: 444412
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.39 ng/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene Symbol5-ME-THF
Alias SymbolsLevomefolic acid; 31690-09-2; LMSR; 5-Methyltetrahydrofolate; Levomefolinic acid
Protein Name5-Methyltetrahydrofolate
Description of Target5-Methyltetrahydrofolate is a methylated derivate of tetrahydrofolate. It is generated by methylenetetrahydrofolate reductase from 5,10-methylenetetrahydrofolate and used to recycle homocysteine back to methionine by 5-methyltetrahydrofolate-homocysteine methyltransferases (also called methionine synthases).