5-Hydroxyindoleacetic acid ELISA Kit
Cat# OKEH02580
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology 5-Hydroxyindoleacetic acid ELISA Kit (OKEH02580) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-5-Hydroxyindoleacetic acid antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 5-Hydroxyindoleacetic acid and incubated. The 5-Hydroxyindoleacetic acid found in the sample or standards competes with the biotinylated 5-Hydroxyindoleacetic acid for limited binding sites on the immobilized anti-5-Hydroxyindoleacetic acid antibody. Excess unbound biotinylated 5-Hydroxyindoleacetic acid and sample or standard 5-Hydroxyindoleacetic acid is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 5-Hydroxyindoleacetic acid captured in the well and inversely proportional to the amount of 5-Hydroxyindoleacetic acid which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.625-40ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 95% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.1% (n = 20) Mean Inter-assay CV%: < 7.5% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: 5HIAA Target Name: 5-Hydroxyindoleacetic acid | ||||||||||||||||||||||
| :: | Pubchem: 1826 | ||||||||||||||||||||||
| :: | Chemical formula: C10H9NO3 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.266 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General 5-Hydroxyindoleacetic acid | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Alias Symbols | 5HIAA, 5-Hydroxyindoleacetic acid, 5HIAA, |
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| Protein Name | 5-Hydroxyindoleacetic acid |
| Description of Target | 5-Hydroxyindoleacetic acid (5-HIAA) is the main metabolite of serotonin in the human body. In chemical analysis of urine samples, 5-HIAA is used to determine the body's levels of serotonin. |