12-Hydroxyeicosatetraenoic acid ELISA Kit
Cat# OKEH02579
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology 12-Hydroxyeicosatetraenoic acid ELISA Kit (OKEH02579) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-12-Hydroxyeicosatetraenoic acid antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 12-Hydroxyeicosatetraenoic acid and incubated. The 12-Hydroxyeicosatetraenoic acid found in the sample or standards competes with the biotinylated 12-Hydroxyeicosatetraenoic acid for limited binding sites on the immobilized anti-12-Hydroxyeicosatetraenoic acid antibody. Excess unbound biotinylated 12-Hydroxyeicosatetraenoic acid and sample or standard 12-Hydroxyeicosatetraenoic acid is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 12-Hydroxyeicosatetraenoic acid captured in the well and inversely proportional to the amount of 12-Hydroxyeicosatetraenoic acid which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 100% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.2% (n = 20) Mean Inter-assay CV%: < 7.3% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: 12HETE Target Name: 12-Hydroxyeicosatetraenoic acid | ||||||||||||||||||||||
| :: | Pubchem: 5283155 | ||||||||||||||||||||||
| :: | Chemical formula: C20H32O3 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.049 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General 12-Hydroxyeicosatetraenoic acid | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Alias Symbols | 12HETE, 12-Hydroxyeicosatetraenoic acid, 12HETE |
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| Protein Name | 12-Hydroxyeicosatetraenoic acid |
| Description of Target | 12-Hydroxyeicosatetraenoic acid (12-HETE) is an eicosanoid. It is a metabolite of arachidonic acid produced by the action of the enzyme arachidonate 12-lipoxygenase. 12-HETE is a highly selective ligand used to label mu opioid receptors. |