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Stemolecule Y27632

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Cat# 04-0012-10

Size : 10mg

Brand : ReproCELL

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Phone : +1 850 650 7790

Stemolecule™ Y27632

04-0012 / 04-0012-10 / 04-0012-02

Brand: Stemolecule

Y27632 is an inhibitor of Rho-associated kinase (ROCK) which is widely used to enhance survival of dissociated PSCs. It is common to supplement cell culture medium with 10 µM of ROCK Inhibit or during cell passage or while establishment of spheroids during the first 24 hours.

CAS Number: 146986-50-7
Alternate Name(s):
  • Y-27632
  • (1R,4R)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexanecarboxamide dihydrochloride

Note: shown do not include shipping and handling charges.

Product Information

Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57: 976-983 (2000).
  • Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K., and Sasai, Y. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25: 681-686 (2007).
  • Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. J Neurosci Res 86: 270-280 (2008).
  • Xu, X., Cowley, S., Flaim, C.J., James, W., Seymour, L.W., Cui, Z. Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation. Biotechnol Prog 26: 781-788 (2010).
  • Fonseca, SAS; Costas, RM; Pereira, LV. Searching for naive human pluripotent stem cells. World Journal of Stem Cells, 7(3), 649 (2015).

    • Additional Publications

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    • Dai S-B; Shen T; Zheng T-T; Pu J-L; Chen X-Z. Plasmid-based generation of neural cells from human fibroblasts using non-integrating episomal vectors. Neural Regen Res 14:510-505 (2019).
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