Stemolecule Wnt Inhibitor IWP-3

Stemolecule Wnt Inhibitor IWP-3 was identified in a high throughput screen for antagonists of the Wnt/β-catenin pathway. Wnt Inhibitor IWP-3 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin¹. The inhibition of the Wnt pathway through the use of IWP-3 has also been shown to promote the formation of cardiomyocytes from human embryonic stem cell-derived mesoderm cells².

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04-0035 Specifications Sheet

Safety Data Sheets:

• 04-0035 Safety Data Sheet

Chen, B., Dodge, M.E., Tang, W., Lu, J., Ma, Z., Fan, C.W., Wei, S., Hao, W., Kilgore, J., Williams, N.S., Roth, M.G., Amatruda, J.F., Chen, C., and Lum, L. Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat Chem Biol 5: 100-107 (2009).

Willems, E., Spiering, S., Davidovics, H., Lanier, M., Xia, Z., Dawson, M., Cashman, J., and Mercola, M. Small-molecule inhibitors of the Wnt pathway potently promote cardiomyocytes from human embryonic stem cell-derived mesoderm. Circ Res 109: 360-364 (2011).

Additional Publications

• Kitano Y; Nishimura S; Kato TM; Ueda A; Takigawa K; Umekaga M; Nomura M; Kawakami A; Ogawa H; Xu H; Hotta A; Takasu N; Tsukahara M. Generation of hypoimmunogenic induced pluripotent stem cells by CRISPR-Cas9 system and detailed evaluation for clinical application. Molecular Therap: Methods Clinical Develop 26:15 (2022).
• Tsujisaka T; Hatani T; Okubo C; Ito R; Kimura A; Narita M; Chonabayashi K ;Funakoshi S; Lucena-Cacace A; Toyoda T; Osafune K; Kimura T; Saito H; Yoshida Y.. Purification of human iPSC-derived cells at large scale using microRNA switch and magnetic-activated cell sorting. Stem Cell Rep 17:1772 (2022).
• Okamoto R; Goto I; Nishimura Y; Kobayashi I; Hashizume R; Yoshida Y; Ito R; Kobayashi Y; Nishikawa M; Ali Y; Saito S; Tanaka T; Sawa Y; Ito M; Dohi K. Gap junction protein beta 4 plays an important role in cardiac function in humans, rodents, and zebrafish. PLos One :240129 (2020).
• Matsura K; Wada M; Sakaguchi K; Matsuhashi Y; Shimizu T. Adequate taylor couette flow-mediated shear stress is useful for dissociating human iPS cell-derived cell aggregates. Regen Therap in press:https://doi.org/10.1016/j.reth.2019.04.006 (2019).
• Ito E; Miyagawa S; Takeda A; Kawamura A; Harada A; Iseoka H; Yajima S; Sougawa N; Michizuki-Oda N; Yasuda S; Sato Y; Sawa Y. Tumorigenicity assay essential for facilitating safety studies of hiPSC-derived cardiomyocytes for clinical application. Sci Rep 9:1881 (2019).
• Lee H; Haller C; Manneville C; Doll T; Fruh I; Gubser Keller C; Richards SM; Ibig-Rehm Y; Patoor M; Goette M; Bouchez LC; Mueller M. Identification of Small Molecules Which Induce Skeletal Muscle Differentiation in Embryonic Stem Cells via Activation of the Wnt and Inhibition of Smad2/3 and Sonic Hedgehog Pathways. Stem Cells 34:299-310 (2016).
• Funakoshi S; Miki K; Takaki T; Okubo C; hatani T; Chonabayashi K; Nishikawa M; Takei I; Oishi A; Narita M; Hoshijima M; Kimura T; Yamanaka S; Yoshida Y. Enhanced engraftment, proliferation, and therapeutic potential in heart using optimized human iPSC-derived cardiomyocytes. Sci Rep 6:19111 (2016).
• Cheesboro J. Mechanism of segmentation in the American cockroach, Perplaneta americana. Ph.D. Thesis, University of Sussex (2012).