Amphotericin B Solution, 100X
Cat# A2230-50ml
Size : 50ml
Brand : US Biological
Grade
Cell Culture GradeShipping Temp
Blue IceStorage Temp
-20°CAbelcet; Abelecet; AmBisome; Ampho-Moronal; Amphocin; Amphozone; Apothecon; Fungilin; Fungizona; Fungizone; Halizon; LNS-AmB; NS 718; NSC 527017
Amphotericin B is a polyene antifungal agent for yeast, mold and mycoplasma. It was first isolated by Gold, et a., from Streptomyces nodosus in 1955. It is an amphoteric compound composed of a hydrophilic polyhydroxyl chain along one side and a lipophilic polyene hydrocarbon chain on the other. Amphotericin B is poorly soluble in water. Amphotericin B binds to sterols, preferentially to the primary fungal cell membrane sterol, ergosterol. This binding disrupts osmotic integrity of the fungal membrane, resulting in leakage of intracellular potassium, magnesium, sugars, and metabolites and then cellular death.
Used in the preparation of baculoviral stocks:
Amphotericin B Solution, 100X is composed of USP grade Amphotericin B in a High pH buffer (NaOH/Na+,H2O). The concentration of Amphotericin B in the final product is 0.25 g/L.
Synonyms:
Abelcet; Abelecet; AmBisome; Ampho-Moronal; Amphocin; Amphozone; Apothecon; Fungilin; Fungizona; Fungizone; Halizon; LNS-AmB; NS 718; NSC 527017
CAS No:
1397-89-3
Molecular Formula:
C47H73NO17
Molecular Weight:
924.09
Appearance:
Light yellow solution
pH:
10-12
Activity:
~250ug/ml
Sterility:
Aseptically prepared by 0.2um filtration
Working Concentration:
Add aseptically 1ml concentrate to 100ml culture medium.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards