Malondialdehyde ELISA Kit

Cat# OKEH02548

Size : 96T

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Malondialdehyde ELISA Kit (OKEH02548) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Malondialdehyde antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Malondialdehyde and incubated. The Malondialdehyde found in the sample or standards competes with the biotinylated Malondialdehyde for limited binding sites on the immobilized anti-Malondialdehyde antibody. Excess unbound biotinylated Malondialdehyde and sample or standard Malondialdehyde is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Malondialdehyde captured in the well and inversely proportional to the amount of Malondialdehyde which was contained in the sample or standard.
ELISA Kit Range0.312-20nmol/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.4% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Malondialdehyde Microplate96 Wells (12 x 8 Well strips)
Malondialdehyde Lyophilized Standard2 x 20 nmol
Sample Diluent1 x 20 mL
100X Malondialdehyde-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: MDA
Target Name: Malondialdehyde
::Pubchem: 10964
::Chemical formula: C3H4O2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.088nmol/mL
SpecificityNatural and recombinant General Malondialdehyde
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Description
Alias SymbolsMDA, Malondialdehyde, MDA
Protein NameMalondialdehyde
Description of TargetMalondialdehyde reacts with deoxyadenosine and deoxyguanosine in DNA, forming DNA adducts, the primary one being M1G, which is mutagenic. The guanidine group of arginine residues condense with MDA to give 2-aminopyrimidines. Malondialdehyde is the organic compound with the formula CH2(CHO)2. The structure of this species is more complex than this formula suggests. This reactive species occurs naturally and is a marker for oxidative stress.

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