Reagents and instruments for immunology, cell biology and molecular biology.
 
> Estradiol ELISA Kit

Estradiol ELISA Kit

Print Print
Please log in

Cat# OKEH02540

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02540
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Estradiol ELISA Kit (OKEH02540) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Estradiol antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Estradiol and incubated. The Estradiol found in the sample or standards competes with the biotinylated Estradiol for limited binding sites on the immobilized anti-Estradiol antibody. Excess unbound biotinylated Estradiol and sample or standard Estradiol is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Estradiol captured in the well and inversely proportional to the amount of Estradiol which was contained in the sample or standard.
ELISA Kit Range3.12-200ng/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.1% (n = 20)
Mean Inter-assay CV%: < 8.0% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Estradiol Microplate96 Wells (12 x 8 Well strips)
Estradiol Lyophilized Standard2 x 1,000 pg
Sample Diluent1 x 20 mL
100X Estradiol-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: E2
Target Name: Estradiol
::Pubchem: 5757
::Chemical formula: C18H24O2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity< 3.12 ng/mL
SpecificityNatural and recombinant General Estradiol
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsE2, Estradiol, E2, oestradiol, 17beta-estradiol
Protein NameEstradiol
Description of TargetEstradiol (E2 or 17β-estradiol, also oestradiol) is a sex hormone. Estradiol is abbreviated E2 as it has 2 hydroxyl groups in its molecular structure. Estrone has 1 (E1) and estriol has 3 (E3). Estradiol is about 10 times as potent as estrone and about 80 times as potent as estriol in its estrogenic effect. The serum levels of estradiol in males (14 - 55 pg/mL) are roughly comparable to those of postmenopausal women (< 35 pg/mL). Estradiol in vivo is interconvertible with estrone; estradiol to estrone conversion being favored. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs, including the bones.