IFNT ELISA Kit (Cattle)

Cat# OKCD04256

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for IFNT ELISA Kit (Cattle) (OKCD04256)
Product Info
Predicted Species ReactivityBos taurus|Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)82-104%93-105%78-89%95-104%
EDTA Plasma (n=5)95-105%96-105%80-104%90-101%
Heparin Plasma (n=5)89-98%80-98%94-103%80-93%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Tau (IFNt). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Tau (IFNt). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Tau (IFNt), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interferon Tau (IFNt) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range7.8-500pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)81-8985
EDTA Plasma (n=5)79-10178
Heparin Plasma (n=5)87-9491
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Tau (IFNt) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Tau (IFNt) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Ifnt Microplate96 Wells (12 x 8 Well strips)
Ifnt Lyophilized Standard2
100X Ifnt HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity3.1 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIFNT2
Gene Full Nameinterferon tau
Alias Symbolsantiluteolysin;IFNT;IFNT1;IFN-tau1;IFN-tau-1;IFN-tau2;IFN-tau-2;IFN-tau-c2;interferon alpha II;interferon tau-1;interferon tau-2;interferon, trophoblast;interferon-tau1e;interferon-tau3e;TP-1;trophoblast antiluteolytic protein;Trophoblast protein 1;trophoblast protein-1;trophoblast type I interferon;trophoblastin.
NCBI Gene Id317698
Protein NameInterferon tau-1
Description of TargetParacrine hormone primarily responsible for maternal recognition of pregnancy. Interacts with endometrial receptors, probably type I interferon receptors, and blocks estrogen receptor expression, preventing the estrogen-induced increase in oxytocin receptor expression in the endometrium. This results in the suppression of the pulsatile endometrial release of the luteolytic hormone prostaglandin F2-alpha, hindering the regression of the corpus luteum (luteolysis) and therefore a return to ovarian cyclicity. This, and a possible direct effect of IFN-tau on prostaglandin synthesis, leads in turn to continued ovarian progesterone secretion, which stimulates the secretion by the endometrium of the nutrients required for the growth of the conceptus. In summary, displays particularly high antiviral and antiproliferative potency concurrently with particular weak cytotoxicity, high antiluteolytic activity and immunomodulatory properties. In contrast with other IFNs, IFN-tau is not virally inducible.
Uniprot IDP15696
Protein Accession #NP_001015511.3
Nucleotide Accession #NM_001015511.3