Iron Assay Kit, BioAssay™

Iron level in blood is a reliable diagnostic indicator of various disease states. Increased levels of iron concentration in blood are associated with blood loss, increased destruction of red blood cells (e.g. hemorrhage) or decreased blood cell survival, acute hepatitis, certain sideroachrestic anemias, ingestion of iron-rich diets, defects in iron storage (e.g. pernicious anemia). Decreased levels of blood iron may result from insufficient iron ingestion from diets, chronic blood loss pathologies, or increased demand on iron storage as during normal pregnancy.

Simple, direct and automation-ready procedures for measuring iron concentrations find wide applications in research, drug discovery and environmental monitoring. Iron assay kit is designed to measure total iron directly in serum without any pretreatment. The improved method utilizes a chromogen that forms a blue colored complex specifically with Fe2+. Fe3+ in the sample is reduced to Fe2+, thus allowing the assay for total iron concentration. The intensity of the color, measured at 590nm, is directly proportional to the iron concentration in the sample.

Sensitive and accurate. Linear detection range 27ug/dL (4.8uM) to 1,000ug/dL (179uM) iron in 96-well plate assay.
Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day.
Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay.
Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples.

Direct Assays: iron in biological samples (e.g. serum).
Drug Discovery/Pharmacology: effects of drugs on iron metabolism.
Environmental Monitoring: iron in soil extracts, mineralized samples.

Kit Contents: (250 tests in 96-well plates)
*I8614-83A: Reagent A: 1x50ml
I8614-83B: Reagent B: 1x4ml
I8614-83C: Reagent C: 1x4ml
I8614-83D: Iron Standard (10mg/dL Fe2+): 1x1ml

Store *I8614-83A: at RT. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.