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> Tacrolimus ELISA Kit

Tacrolimus ELISA Kit

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Cat# OKEH02586

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02586
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Tacrolimus ELISA Kit (OKEH02586) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Tacrolimus antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tacrolimus and incubated. The Tacrolimus found in the sample or standards competes with the biotinylated Tacrolimus for limited binding sites on the immobilized anti-Tacrolimus antibody. Excess unbound biotinylated Tacrolimus and sample or standard Tacrolimus is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tacrolimus captured in the well and inversely proportional to the amount of Tacrolimus which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/ml
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.7% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Tacrolimus Microplate96 Wells (12 x 8 Well strips)
Tacrolimus Lyophilized Standard2 x 100 ng
Sample Diluent1 x 20 mL
100X Tacrolimus-Biotin Complex1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: FK506
Target Name: Tacrolimus
::Pubchem: 445643
::Chemical formula: C44H69NO12
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.069 ng/mL
SpecificityNatural and recombinant General Tacrolimus
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsTacrolimus, FK506, fujimycin
Protein NameTacrolimus
Description of TargetTacrolimus (also FK-506 or fujimycin, trade names Prograf, Advagraf, Protopic) is an immunosuppressive drug that is mainly used after allogeneic organ transplant to reduce the activity of the patient's immune system and so lower the risk of organ rejection. It is also used in a topical preparation in the treatment of atopic dermatitis (eczema), severe refractory uveitis after bone marrow transplants, exacerbations of minimal change disease, and the skin condition vitiligo. It is a 23-membered macrolide lactone discovered in 1984 from the fermentation broth of a Japanese soil sample that contained the bacteria Streptomyces tsukubaensis. It reduces interleukin-2 (IL-2) production by T-cells.