T7 High Efficiency Transcription Kit
Cat# JT101-01
Size : 20ul×25rxns
Brand : TransGen Biotech
T7 High Efficiency Transcription Kit is designed for in vitro RNA synthesis by T7 RNA Polymerase with supercoiled or linearized DNA templates. More than 50 μg of RNA can be produced from a 20 μl reaction with 1 μg of template added. Synthesized RNA can be used for in vitro translation, RNase protection assays, RNA splicing, and hybridization assays.
Storage
at -20°C for one year
Shipping
Dry ice (-70 ℃)
Component | JT101-01 (25 rxns) |
T7 Transcription Enzyme Mix | 50 μl |
5×T7 Transcription Reaction Buffer | 100 μl |
10 mM NTP Mix | 200 μl |
DNase I (1 unit/μl) | 25 μl |
500 mM EDTA (pH 8.0) | 25 μl |
RNase-free Water | 500 μl |
Control Transcription Template (0.5 μg/μl) | 10 μl |
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