PSA Total ELISA Kit

Specifications

Product Data
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	0 - 25 ng/mL
Sample Type	Cell culture supernatant, serum, plasma
Sample Volume	25 ul
Specificity	Natural and recombinant human PSA Total
Reactivities	Human
Interference	No significant interference observed with available related molecules.
Components	See the product information for more details
Background	This assay is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with an antibody, directed towards an epitope of an antigen molecule (PSA). An aliquot of specimen sample serum is incubated in the coated well with enzyme conjugated second antibody (E-Ab), directed towards a different region of the antigen molecule. After incubation the unbound E-Ab is washed off. The amount of bound E-Ab is proportional to the concentration of antigen in the sample. After adding the substrate solution, the intensity of color developed is proportional to the antigen concentration in the sample. The measured ODs of the standards are used to construct a calibration curve against which the unknown samples are calculated.