Precerebellin (CBLN1) (NM_004352) Human Tagged ORF Clone
CAT#: RC210797
CBLN1 (Myc-DDK-tagged)-Human cerebellin 1 precursor (CBLN1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
Product Images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Precerebellin |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | >RC210797 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGCGTCCTGGAGCTGCTGCTGCTGGGGGCTGCGTGGCTGGCGGGCCCGGCCCGCGGGCAGAATG AGACGGAGCCCATCGTGCTGGAGGGCAAGTGCCTGGTGGTGTGCGACTCCAACCCCACGTCCGACCCCAC GGGCACTGCCCTGGGCATCTCTGTGCGCTCTGGCAGCGCCAAGGTGGCTTTCTCTGCCATCAGGAGCACC AACCACGAGCCGTCCGAGATGAGTAATCGCACCATGATCATCTACTTCGACCAGGTACTAGTGAACATTG GGAACAACTTTGATTCAGAACGCAGCACTTTCATCGCCCCGCGCAAAGGGATCTACAGTTTTAACTTCCA CGTGGTAAAAGTCTACAACAGACAAACCATACAGGTGAGCCTCATGCTAAACGGGTGGCCGGTGATTTCA GCCTTCGCTGGTGACCAGGACGTGACCCGGGAGGCCGCCAGCAACGGAGTCCTAATCCAAATGGAGAAAG GCGACCGAGCATACCTCAAGCTGGAGCGGGGAAACTTGATGGGGGGCTGGAAGTACTCGACCTTCTCCGG ATTCCTGGTGTTTCCTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210797 protein sequence Red=Cloning site Green=Tags(s) MLGVLELLLLGAAWLAGPARGQNETEPIVLEGKCLVVCDSNPTSDPTGTALGISVRSGSAKVAFSAIRST NHEPSEMSNRTMIIYFDQVLVNIGNNFDSERSTFIAPRKGIYSFNFHVVKVYNRQTIQVSLMLNGWPVIS AFAGDQDVTREAASNGVLIQMEKGDRAYLKLERGNLMGGWKYSTFSGFLVFPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | CHROMATOGRAMS Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_004352 |
| ORF Size | 579 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004352.4 |
| RefSeq Size | 2435 bp |
| RefSeq ORF | 582 bp |
| Locus ID | 869 |
| UniProt ID | P23435 |
| Cytogenetics | 16q12.1 |
| Protein Families | Secreted Protein |
| MW | 21.1 kDa |
| Gene Summary | This gene encodes a cerebellum-specific precursor protein, precerebellin, with similarity to the globular (non-collagen-like) domain of complement component C1qB. Precerebellin is processed to give rise to several derivatives, including the hexadecapeptide, cerebellin, which is highly enriched in postsynaptic structures of Purkinje cells. Cerebellin has also been found in human and rat adrenals, where it has been shown to enhance the secretory activity of this gland. [provided by RefSeq, Aug 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| RC210797L1 | Lenti ORF clone of Human cerebellin 1 precursor (CBLN1), Myc-DDK-tagged | 600,00 EUR | |
| RC210797L2 | Lenti ORF clone of Human cerebellin 1 precursor (CBLN1), mGFP tagged | 600,00 EUR | |
| RC210797L3 | Lenti ORF clone of Human cerebellin 1 precursor (CBLN1), Myc-DDK-tagged | 600,00 EUR | |
| RC210797L4 | Lenti ORF clone of Human cerebellin 1 precursor (CBLN1), mGFP tagged | 600,00 EUR | |
| RG210797 | CBLN1 (tGFP-tagged) - Human cerebellin 1 precursor (CBLN1) | 500,00 EUR | |
| SC303471 | CBLN1 (untagged)-Human cerebellin 1 precursor (CBLN1) | 330,00 EUR |