Pnpt1 (NM_001142371) Rat Tagged ORF Clone

CAT#: RR201251

  • TrueORF®

Pnpt1 (Myc-DDK-tagged ORF) - Rat polyribonucleotide nucleotidyltransferase 1 (Pnpt1), (10 ug)

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

Datasheet
SDS
Plasmid Map

Product Images

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Pnpt1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR201251 representing NM_001142371
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTTGCAGGCCGTGCTGCTTGTGCCTGTGTCTCCGACCGCTGAGCTGCGGTCCCCTCGGCCGGC
CAAGGCGGCATCGTGCACTCAGCTATTTGCAGGTGCGAGCGCTGTGGAGCGGCACCCGGTCCAGAGCAGT
GACTGTGGACTTGGGACACAGAAAATTAGAAATATCCTCTGGAAAACTGGCAAGATTTGCTGATGGCTGT
GCTGTGGTACAGTCAGGTGATACTGCAGTAATGGTCACAGCGGTCAGCAAAACAAAACCTTCACCTTCCC
AATTCATGCCATTGGTGGTAGACTACAGACAGAAGGCTGCTGCAGCAGGTAGAATCCCCACAAACTACCT
TAGAAGAGAGATTGGCTCTTCTGACAAAGAGATTCTTACAAGTCGAGTAATAGATCGTTCAATTCGACCT
CTCTTTCCTGCTGGCTATTTTTATGATACTCAGGTTCTCTGTAATCTGCTAGCAGTAGATGGCATCAATG
AACCTGATATCTTAGCAATTAATGGCGCTTCTGCAGCTCTCTCCTTATCAGATATCCCTTGGAATGGACC
TGTTGGGGCAGTACGAATAGGAATGATCGATGGAGAATGTGTCGTTAACCCAACAAGGAAAGAAATGTCT
TCTAGCACCTTAAATTTAGTAGTTGCTGGAGCACCTAAAAGCCAAATTGTTATGTTGGAAGCCTCTGCAG
AAAACATTTTACAGCAGGACTTTTGCCATGCTATCAAAGTCGGGGTGAAGTATACGCAGCAGATAATTCA
GAGCATCCAGCAGTTGGTAAAAGAAATCGGTGTTGCCAAGAGGACACCCCAGAAGATATTTACTCCTTCT
GCAGAGATTGTGAAATACACGCATACAATCGCCATGGAGAAACTCTATGCAGTTTTTACAGATTATGAAC
ATGATAAAGTTTCCAGGGATGAAGCTGTTAACAAGATAAGATTAGATACAGAGGAACATCTAAAGGAAAA
ATTTCCAGAGGTTGACCAACTTGAAATAATAGAATCCTTCAACGTTGTTGCAAAGGAGGTTTTCAGAAGT
ATTATTTTGAATGAATACAAAAGGTGTGATGGACGAGATCTGACTTCACTTAGGAATATAAGTTGCGAGG
TAGATATGTTTAAAACACTTCATGGATCAGCATTATTTCAGAGAGGACAAACACAGGTACTCTGTACTGT
TACATTTGATTCATTAGAATCCAGTATTAAATCAGATCAAATTATAACAGCTATAAATGGGGTAAAAGAT
AAAAATTTCATGTTGCACTATGAGTTTCCTCCTTATGCAACCAATGAAACTGGCAAAGTTACTGGTGTAA
ATAGAAGAGAACTTGGACATGGTGCTCTTGCTGAGAAAGCTTTGTGTCCTGTTATTCCCAAAGATTTTCC
TTTTACCATAAGAGTTACATCTGAAGTTCTCGAGTCAAACGGGTCATCTTCTATGGCATCTGCATGTGGT
GGAAGTTTGGCATTAATGGATGCAGGGGTCCCAATTTCATCTGCTGTTGCAGGTGTAGCAGTGGGATTGG
TTACCAAAAGCAATCCAGAGAAAGGTGAAATAGAAGACTATCGTTTGCTAACAGATATTCTGGGAATTGA
AGATTACAATGGTGACATGGATTTCAAAATAGCTGGTACAAATAAAGGAATAACTGCATTACAGGCTGAT
ATTAAGTTACCTGGAGTACCAGTTAAAATTATAATGGAAGCCATCCAACAAGCAACAGTGGCAAAGAGGG
AAATATTGCAGACAATGAGCAAAACGATTTCAAAACCTCGAGCATCTAGGAAAGAAAATGGACCAGTTGT
AGAAACAGTAAAGGTTCCATTATCAAAACGAGCAAAATTCATTGGGCCTGGTGGATATCACTTAAAAAAA
CTCCAGGCTGAGACAGGTGTAACAATTAGTCAGGTTGATGAAGAAACATTCTCCATATTTGCACCAACAC
CTACTGCAATGCATGAGGCAAGAGACTTCATTACAGAAATTTGCAGAGATGATCAAGAGCAACAATTGGA
ATTTGGAGCAGTTTATACCGCTACAATAACTGAAATCAGAGACACAGGAGTAATGGTAAAACTGTATCCA
AACATGACTGCAGTGCTACTTCATAATTCACAACTTGACCAACGAAAGATTAAACATCCCACTGCCCTGG
GATTAGAAGTTGGCCAGGAAATTCAGGTCAAATACTTTGGCCGTGATCCAGCTGATGGAAGAATGAGGCT
TTCCCGTAAAGTACTTCAGTCTCCAGCTACAACTGTTCTCAAAACTCTAAATGATAGGAGCAGCATTGTA
ATGGGAGAGCCTATTTCACAGTCATCATCTAACTCTTCCCCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RR201251 representing NM_001142371
Red=Cloning site Green=Tags(s)

MAACRPCCLCLCLRPLSCGPLGRPRRHRALSYLQVRALWSGTRSRAVTVDLGHRKLEISSGKLARFADGC
AVVQSGDTAVMVTAVSKTKPSPSQFMPLVVDYRQKAAAAGRIPTNYLRREIGSSDKEILTSRVIDRSIRP
LFPAGYFYDTQVLCNLLAVDGINEPDILAINGASAALSLSDIPWNGPVGAVRIGMIDGECVVNPTRKEMS
SSTLNLVVAGAPKSQIVMLEASAENILQQDFCHAIKVGVKYTQQIIQSIQQLVKEIGVAKRTPQKIFTPS
AEIVKYTHTIAMEKLYAVFTDYEHDKVSRDEAVNKIRLDTEEHLKEKFPEVDQLEIIESFNVVAKEVFRS
IILNEYKRCDGRDLTSLRNISCEVDMFKTLHGSALFQRGQTQVLCTVTFDSLESSIKSDQIITAINGVKD
KNFMLHYEFPPYATNETGKVTGVNRRELGHGALAEKALCPVIPKDFPFTIRVTSEVLESNGSSSMASACG
GSLALMDAGVPISSAVAGVAVGLVTKSNPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQAD
IKLPGVPVKIIMEAIQQATVAKREILQTMSKTISKPRASRKENGPVVETVKVPLSKRAKFIGPGGYHLKK
LQAETGVTISQVDEETFSIFAPTPTAMHEARDFITEICRDDQEQQLEFGAVYTATITEIRDTGVMVKLYP
NMTAVLLHNSQLDQRKIKHPTALGLEVGQEIQVKYFGRDPADGRMRLSRKVLQSPATTVLKTLNDRSSIV
MGEPISQSSSNSSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001142371
ORF Size 2352 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001142371.1, NP_001135843.1
RefSeq Size 3441 bp
RefSeq ORF 2355 bp
Locus ID 360992
Cytogenetics 14q22
MW 85.9 kDa

Other Versions

SKU Description Size
RN201251 Pnpt1 (untagged ORF) - Rat polyribonucleotide nucleotidyltransferase 1 (Pnpt1), (10 ug)
    • 10 ug

791,00 EUR

RR201251L3 Lenti ORF clone of Pnpt1 (Myc-DDK-tagged ORF) - Rat polyribonucleotide nucleotidyltransferase 1 (Pnpt1), (10 ug)
    • 10 ug

1 090,00 EUR

RR201251L4 Lenti ORF clone of Pnpt1 (mGFP-tagged ORF) - Rat polyribonucleotide nucleotidyltransferase 1 (Pnpt1), (10 ug)
    • 10 ug

1 090,00 EUR