Nutrient Mixture Ham’s F-10 w/HEPES, w/o L-Glutamine (Powder)

Cat# N8540-05-25L

Size : 25L

Brand : US Biological

Request more information

Contact local distributor :


Phone : +1 850 650 7790


N8540-05 Nutrient Mixture Ham’s F-10 w/HEPES, w/o L-Glutamine (Powder)

Clone Type
Polyclonal
Grade
Cell Culture Grade
Shipping Temp
RT
Storage Temp
RT/4°C

Ham’s Nutrient Mixtures were originally developed to support clonal growth of several clones of Chinese hamster ovary (CHO) cells, as well as clones of HeLa and mouse L-cells. Both mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham’s F-10 has been shown to support the growth of human diploid cells, white blood cells for chromosomal analysis, primary explants of rat, rabbit and chicken tissues. Ham’s F-12 has been used for the growth of primary rat hepatocytes and rat prostate epithelial cells. May be used in combination with hormones and transferrin. For optimum buffering, use in a 5% CO2 atmosphere.

Appearance:
White to light pink, homogenous, free flowing powder

Solubility:
Light yellow to yellow, clear, complete

pH:
As Reported

Endotoxin:
≤1EU/ml

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Ham, R.G., Exp. Cell Res. 29: 515-526. (1963). 2. Ham, R.G., Proc. Nat. Acad. Sci. 53: 288-293. (1965).