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> M91_08174 Chemi-Luminescent ELISA Kit (Bovine)

M91_08174 Chemi-Luminescent ELISA Kit (Bovine)

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Cat# OKCD03310

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for M91_08174 Chemi-Luminescent ELISA Kit (Bovine) (OKCD03310)
Product Info
Predicted Species ReactivityBos mutus|Bovine
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)89-104%80-98%99-105%82-96%
EDTA Plasma (n=5)80-91%81-99%86-97%92-99%
Heparin Plasma (n=5)79-94%80-96%82-95%78-99%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Amphiregulin (AREG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Amphiregulin (AREG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Amphiregulin (AREG) level in the sample or standard.
ELISA Kit Range27.4-20,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)90-10498
EDTA Plasma (n=5)95-10599
Heparin Plasma (n=5)88-9691
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amphiregulin (AREG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amphiregulin (AREG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
M91_08174 Microplate96 Wells (12 x 8 Well strips)
M91_08174 Lyophilized Standard2
100X M91_08174 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.312 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAREG
Gene Full Nameamphiregulin
Alias Symbolsamphiregulin.
NCBI Gene Id102270841
Protein NameAmphiregulin
Description of TargetAmphiregulin
Uniprot IDL8IUA9
Protein Accession #XP_005891318
Nucleotide Accession #XM_005891256