Lats1 Mouse Gene Knockout Kit (CRISPR)
CAT#: KN309147
Lats1 - mouse gene knockout kit via CRISPR, HDR mediated
Functional Cassette: Luciferase-Puro RFP-BSD mBFP-Neo
HDR-mediated knockout kit validation
Product Images
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control |
| Donor DNA | GFP-puro |
| Symbol | Lats1 |
| Locus ID | 16798 |
| Components | KN309147G1, Lats1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AGAGGGGTGAAAAGCCAGAA KN309147G2, Lats1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGGAGATCCGAGAATCCCTG KN309147D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences: pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CACTTAGTAT CCTTTATGAT TGTATAAGCC TTACTTTCTG AAGCAGTTAG AAGGTTTGTT TGTTTGTTTG TTTATTTATT TATTTGTCAC AAATGCTGCT AGTTAGAAAT ATTGTTTTTT GGGTTTGGGT TTTTTTTTTT TTTTTTTCCC CGAGACAGGG TTTCTCTGTG TAGCTCTGGC TGTCCTGGGA CTCACTCTGT AGACCAGGCT GGCCTCAAAC TCAGAAATCC GCCTGCCTCT GCCTCCCAAG TGCTGAGATT AAAGGAATGC ACCACCACTG TCCGGCTAGA AATATTGTAA ATCCACTTTA GAAACTAGGA AATTGGCACA GAGAGGTGTA GTAACTTACC TTGATCCTAC AGTACTATGT CATGAAGTCT GAGCAAGCAA CTCCCATAGC AGTGGGGGTT TCCCCTAATA CAACGAACTG CTTCATTAAT AACTCTAGCT GTGTATTTTC TTTCCTAGGT TAGTTACTTC CACTGTAAAG GATTTTAACA TTCCTGGGAC TTCCCCTTTC ACCTTGGCTC AGAAGAAGCT CTGAGTCTTT GAGTTAGCTC CTTAAGAAGT GCTTTGCAGG GGCCACATAT AGATGCTTTC AAGGCCGAGC ATAACCTGAA TAAGATGTCG ACTGAAGATC CCAGGCAGGT GAGAAATCCA CCCAAATTTG GCACACATCA TAAAGCCTTG CAAGAAATTC GAAACTCTCT ACTACCATTT GCAAATGAAA CAAGTTCTTC CCGGAGCCCT TCAGAAGTTA ATCCACAGAT GTTTCAGGAT TTGCAGGCTG CTGGCTTTGA TGAGGTAAGA ACTTTTAAAA ACAAAAATAA AAGCAAACAA ACAAACACCA TCACAAATGG TTTTTCTTAT TCTGTGAGGC AAACAAGTTC AAGGGGAGCT CATAAGGGCG TTTCTTTTGA GAGGACTTAC CTGTACATTT AAGTTTGTGT GTTCCATTAA CAGAGAATAC TACCACAGTA ATACTCTTAC TAACTAGTAT GACAGAATGA TTGTTATTGC CTTAGTTTCT TCTGTGCTAT CCCCAAAGCC TGGAGTTTCT GTTGGTGAGG TCATGAAACC AGAGTGGAGA TGCAGTTGAG GGAGGCTGCA AGTGAGGATG GACGGGCTTG GTCGCAGCCT GAGATATAGA CACTGATCTC AGCTGGTGTT GAGAGTGAGA GTGCCATGGC TGTGAAGTCTGE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_010690 |
| UniProt ID | Q8BYR2 |
| Synonyms | AW208599 |
| Summary | Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cells differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| KN309147BN | Lats1 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN309147LP | Lats1 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN309147RB | Lats1 - mouse gene knockout kit via CRISPR, HDR mediated | 1 657,00 EUR | |
| KN509147 | Lats1 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 657,00 EUR | |
| GA202419 | Lats1 CRISPRa kit - CRISPR gene activation of mouse large tumor suppressor | 1 657,00 EUR |