Hydroxychloroquine [118-42-3]
Cat# HY-W031727-500mg
Size : 500mg
Brand : MedChemExpress
Hydroxychloroquine (HCQ) is a synthetic oral antimalarial drug that can be used in the study of malaria and autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis. Hydroxychloroquine is a potent autophagic flux inhibitor with antiviral activity (such as SARS-CoV-2 virus) that inhibits Toll-like receptor 7/9 (TLR7/9) signaling.
For research use only. We do not sell to patients.
Hydroxychloroquine Chemical Structure
CAS No. : 118-42-3
This product is a controlled substance and not for sale in your territory.
Based on 49 publication(s) in Google Scholar
Other Forms of Hydroxychloroquine:
- Hydroxychloroquine sulfate In-stock
- Hydroxychloroquine-d4-1 sulfate Get quote
- Hydroxychloroquine-d5 Get quote
View All Parasite Isoform Specific Products:
View All Toll-like Receptor (TLR) Isoform Specific Products:
Description |
Hydroxychloroquine (HCQ) is a synthetic oral antimalarial drug that can be used in the study of malaria and autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis. Hydroxychloroquine is a potent autophagic flux inhibitor with antiviral activity (such as SARS-CoV-2 virus) that inhibits Toll-like receptor 7/9 (TLR7/9) signaling[1][2][3][4][5][6][7][8]. |
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IC50 & Target |
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In Vitro |
Hydroxychloroquine ( 0-100 μM, 48h) can inhibit the activity of SARS-CoV-2 virus in Vero E6 cells[4]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis[5]
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In Vivo |
Hydroxychloroquine (HCQ) (25 μM, Intraperitoneal injection (i.p.), Once a week for two weeks) has the effect of reducing the survival rate of endometriosis cells and the number of lesions and histopathology in mouse models of endometriosis[6]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Clinical Trial |
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Molecular Weight |
335.87 |
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Formula |
C18H26ClN3O |
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CAS No. |
118-42-3 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
ClC1=CC=C2C(NC(CCCN(CCO)CC)C)=CC=NC2=C1 |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
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Solvent & Solubility |
In Vitro:
DMSO : ≥ 100 mg/mL (297.73 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) 1M HCl : 100 mg/mL (297.73 mM; adjust pH to 2 with HCl) H2O : 1.67 mg/mL (4.97 mM; ultrasonic and warming and heat to 60°C) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: ≥98.0% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O / DMSO / 1M HCl | 1 mM | 2.9773 mL | 14.8867 mL | 29.7734 mL | 74.4336 mL |
DMSO / 1M HCl | 5 mM | 0.5955 mL | 2.9773 mL | 5.9547 mL | 14.8867 mL |
10 mM | 0.2977 mL | 1.4887 mL | 2.9773 mL | 7.4434 mL | |
15 mM | 0.1985 mL | 0.9924 mL | 1.9849 mL | 4.9622 mL | |
20 mM | 0.1489 mL | 0.7443 mL | 1.4887 mL | 3.7217 mL | |
25 mM | 0.1191 mL | 0.5955 mL | 1.1909 mL | 2.9773 mL | |
30 mM | 0.0992 mL | 0.4962 mL | 0.9924 mL | 2.4811 mL | |
40 mM | 0.0744 mL | 0.3722 mL | 0.7443 mL | 1.8608 mL | |
50 mM | 0.0595 mL | 0.2977 mL | 0.5955 mL | 1.4887 mL | |
60 mM | 0.0496 mL | 0.2481 mL | 0.4962 mL | 1.2406 mL | |
80 mM | 0.0372 mL | 0.1861 mL | 0.3722 mL | 0.9304 mL | |
100 mM | 0.0298 mL | 0.1489 mL | 0.2977 mL | 0.7443 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.