Human GRP78 (Glucose Regulated Protein 78) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	HSPA5
Gene ID:	3309
Uniprot ID:	BIP_HUMAN
Specificity:	This kit recognizes Human GRP78 in samples. No significant cross-reactivity or interference between Human GRP78 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1. Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation. (Microbial infection) Plays an important role in viral binding to the host cell membrane and entry for several flaviruses such as Dengue virus, Zika virus and Japanese encephalitis virus. Acts as a component of the cellular receptor for Dengue virus serotype 2/DENV-2 on human liver cells. (Microbial infection) Acts as a receptor for CotH proteins expressed by fungi of the order mucorales, the causative agent of mucormycosis, which plays an important role in epithelial cell invasion by the fungi. Acts as a receptor for R.delemar CotH3 in nasal epithelial cells, which may be an early step in rhinoorbital/cerebral mucormycosis (RCM) disease progression.
Protein Name	Endoplasmic Reticulum Chaperone Bip 78 Kda Glucose-Regulated Protein Grp-78 Binding-Immunoglobulin Protein Bip Heat Shock Protein 70 Family Protein 5 Hsp70 Family Protein 5 Heat Shock Protein Family A Member 5 Immunoglobulin Heavy Chain-Binding Protein
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-3371453 Reactome: R-HSA-381033 Reactome: R-HSA-381042 Reactome: R-HSA-381070 Reactome: R-HSA-381183 Reactome: R-HSA-8874177 Reactome: R-HSA-983170
Cellular Localisation	Endoplasmic Reticulum Lumen Melanosome Cytoplasm Cell Surface Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv Localizes To The Cell Surface Of Epithelial Cells In Response To High Levels Of Free Iron
Alternative ELISA Names	Endoplasmic Reticulum Chaperone Bip ELISA kit 78 Kda Glucose-Regulated Protein ELISA kit Grp-78 ELISA kit Binding-Immunoglobulin Protein ELISA kit Bip ELISA kit Heat Shock Protein 70 Family Protein 5 ELISA kit Hsp70 Family Protein 5 ELISA kit Heat Shock Protein Family A Member 5 ELISA kit Immunoglobulin Heavy Chain-Binding Protein ELISA kit HSPA5 ELISA kit GRP78 ELISA kit
Specificity	This kit recognizes Human GRP78 in samples. No significant cross-reactivity or interference between Human GRP78 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-107	99
EDTA plasma (n=8)	89-102	96
Cell culture media (n=8)	88-103	95

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	1.99	4.19	19.92	2.04	4.48	18.55
Standard deviation	0.13	0.18	1.10	0.10	0.19	0.64
CV (%)	6.53	4.30	5.52	4.90	4.24	3.45

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance