HLA DM (HLA-DMA) rat monoclonal antibody, clone M5/114, Azide Free
Specifications
Product Data
Clone Name
M5/114
Application
FC, FN, IHC, IP, WB
Recommended Dilution
Flow Cytometry: 2 μg/ml. Immunoprecipitation. Western blot. Immunohistochemistry on Paraffin Sections. Immunohistochemistry on Frozen Sections. Functional Application: Blocking of T cell proliferative responses.
Reactivity
Mouse
Antibody Host
Rat
Isotype
IgG2b
Clonality
Monoclonal
Immunogen
Activated C57BL/6 mouse spleen cells
Specificity
The antibody reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
